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News and Highlights

  • OriGene secures $16 million in series B funding - March 17, 2010 [>>]
  • OriGene Technologies and Essen Instruments to co-develop ion channel cell lines - March 15, 2010 [>>]
  • Luminex assays for miRNA, transcription factors, and SNP genotyping [>>]
  • OriGene launches comprehensive reverse-phase protein array for cancer biomarker research [>>]
  • OriGene launches 5,000 human proteins expressed in human HEK293 cells. [>>]
  • OriGene launches 649 human miRNA and 498 mouse miRNA for functional study. [>>]

Meet Us at These Events

US HUPO Proteomic
March 7-10, 2010, Denver, CO, Booth 18

AACR Annual Meeting 2010
April 17-21, 2010, Washington, DC, Booth 1027

Immunology Annual Meeting 2010
May 7-11, 2010, Baltimore, MD

Planet xMAP USA 2010
May 12-14, 2010, Baltimore, MD

ASMS 2010
May 23-27, 2010, Salt Lake City, UT

Product Overview

cDNA clones: Over-expression

• TrueORF Gold: Tagged cDNA Clones (New)
- Tested individually by Western
- Sequence verified
- Next day delivery
- Transfection ready
- Easily shuttled into over 60 vectors

• TrueORF: Tagged cDNA Clones
- 11,000 with Western.
- GFP, RFP, YFP vectors
- Over 50 Organelle Markers
• TrueClone: Native protein expression
• Gene Synthesis

• MicroRNA expression plasmids

RNAi: Down-regulation

• Pre-designed HuSH-29 shRNA
- Every human, mouse and rat genes
- In retroviral, GFP and RFP vectors
- 4 constructs per gene
- Guaranteed knock down
• Custom Exact-shRNA service
• MicroRNA

• TissueScan cancer qPCR arrays
• Genome wide qPCR primer pairs
• qPCR template standards
• Human cancer tissue products

• 5,000 purified human proteins (New)
• 5000 primary antibodies
• VERIFY Tagged AntigenTM
(over-expression lysate)

Biomarker validation & SNP Analysiscancer

OriGene has the largest human cancer biorepository for biomarker discovery and validation. Since the year 2000 the company has invested over $20 million in this biorepository, resulting in over 140,000 human biospecimens representing over 12,000 donor cases.

TissueScan Cancer qPCR Arrays are panels of normalized cDNA prepared from pathologist-verified human tumor tissues. With a gene-specific qPCR, TissueScan reveals expression changes of an individual gene across a large number of disease stages, providing quick and comprehensive information on expression profiles or SNP status.

 

  • High quality cancer tissues – From accredited medical institutions in US under highest bioethical guidelines
  • Comprehensive information – Detailed pathology reports with tumor staging, HE images, etc
  • Broad tumor coverage – Multiple panels for breast, colon, lung, ovarian, and prostate cancers
  • Convenient and fast – qPCR ready format; results in less than 2 hours.

trueORF_GOldTrueORF Gold is OriGene’s collection of premium cDNA clones that have passed the ultimate tests: sequence verification and protein expression validation. Why settle for clones with little validation or wait for gene-synthesis? Get a TrueORF Gold clone and start your experiment immediately.

Comparison of cDNA clones from different sources

Sequence-Verification

Expression Validation

Wait Time

Shuttling into 60 vectors
trueORF_GOld

Yes

Yes

None

Yes

Clones constructed in your lab

Y/N

Y/N

Varies

No

Clones obtained from other vendors

Y/N

No

Varies

No

Clones obtained by gene-synthesis

Yes

No

Long

No

OriGene offers a broad selection of monoclonal and polyclonal antibodies to human proteins for various applications, among them

  • >3,500 antibodies include a tube of gene specific over-expression cell lysate as a positive control
  • Target important human proteins covering various pathways
  • >300 TrueMAB™ antibodies are made with authentic protein immunogens to provide superior performance recognizing native epitopes
  • Anti-tag antibodies for the detection of recombinant proteins
  • Validated for various immuno-applications with money back guarantee

product_overview

Powered by extensive collection of TrueORF human cDNA clones, OriGene now offers >5,000 full length human proteins expressed in HEK293 cells and purified with affinity column.

  • The broadest offering of human proteins produced in human cells
  • Optimal preservation of protein structure, post-translational modifications and functions
  • >80% purity through affinity purification
  • Options for large scale production available.

Applications

  • Native antigens for optimized antibody production
  • Positive controls in antibody assays
  • Standards in ELISA and other assays
  • Protein-protein interaction
  • In vitro biochemical assays and cell-based functional assays

landing

HuSH-29 are pre-designed shRNA with genome wide coverage (human, mouse and rat) that delivers guaranteed knockdown. The same superior design is also offered for other species through Exact-shRNA.

(Learn more about HuSH-29...)

Features and Benefits

  • Three retroviral vector options:
    • pRS: basic retroviral vector without fluorescence
    • pRS with GFP to monitor transfection
    • pRS with RFP, ideal for double knockdown
  • Guaranteed successful gene knockdown (≥70%)
  • Versatile applications: transient or stable transfection or retroviral infection
  • As low as $595 for a kit (4 gene-specific shRNA and two negative controls)

Double Gene Knockdown Experiment

KnockDown

Scrambled shRNA (GFP Vector)
+
Scrambled shRNA (RFP Vector)

shRNA against RFP (GFP Vector)
+
shRNA against GFP (RFP Vector)

TrueORF cDNA clones are tagged cDNA clones for protein studies.

  • 25,000 human and 12,000 mouse ORF clones
  • 11,000 clones already validated by Western blot
    (see example)
  • Two types of ready-to-order clones:
    Myc-DDK tagged or GFP tagged
  • 40 destination vectors for additional applications
  • Easy shuttling with RapidShuttling kit.
  • 10 μg transfection-ready DNA using PowerPrep® HP Kits

New: Learn about TrueORF and PrecisionShuttle from a 15 min audio slide show.

Intro3